Phytofabrication of biomolecule-coated metallic silver nanoparticles using leaf extracts of in vitro-raised bamboo species and its anticancer activity against human PC3 cell lines

Authors : KALAMEGAM KALAIARASI, GOVINDARAJ PRASANNARAJ, SHIVENDRA VIKRAM SAHI, PERUMAL VENKATACHALAM

Pages : 223-232

Doi:10.3906/biy-1406-10

View : 25 | Download : 13

Publication Date : 2015-12-01

Article Type : Research Paper

Abstract :The present study describes the biosynthesis of metallic silver nanoparticles insert ignore into journalissuearticles values(AgNPs); from silver ions using in vitro-grown leaf samples of Bambusa arundinacea insert ignore into journalissuearticles values(Ba); and Bambusa nutans insert ignore into journalissuearticles values(Bn); and their anticancer activity against human prostatic cancer cell lines insert ignore into journalissuearticles values(PC3);. The metallic AgNPs were synthesized at room temperature by treating the leaf extracts of Bambusa arundinacea and Bambusa nutans with 2 mM silver nitrate using the boiled method. The characterization of synthesized BaAgNPs and BnAgNPs was performed using UV-Vis spectrum insert ignore into journalissuearticles values(absorption peak at 365 nm);, FTIR, TEM insert ignore into journalissuearticles values(spherical shape);, SEM insert ignore into journalissuearticles values(size of 30-90 nm);, and EDX analysis. Cell viability assays were carried out to determine the cytotoxicity effect of AgNPs on the PC3 cancer cell line and the Vero normal cell line. The IC_50 values for BaAgNP- and BnAgNP-treated PC3 cancer cells were found to be 73.57 µg/mL and 84.88 µg/mL while the values for treated Vero cells were found to be 93.58 µg/mL and 96.41 µg/mL, respectively. The percentages of the apoptotic bodies by AO/EtBr staining were found to be 76% and 62% for BaAgNPs and BnAgNPs, respectively. The results strongly suggest that synthesized BaAgNPs show potential anticancer activity against human PC3 cell lines compared to BnAgNPs.
Keywords : Anticancer activity, biological synthesis, bamboo, silver nanoparticles, electron microscopy, PC3 human prostate cancer cell lines