Amelioration of subchronic acrylamide toxicity in large intestine of rats byorganic dried apricot intake

Authors : Mehmet Erman ERDEMLİ, Zümrüt DOĞAN, Yilmaz ÇİĞREMİŞ, Müslüm AKGÖZ, Eyüp ALTINÖZ, Murat GEÇER, Yusuf TÜRKÖZ

Pages : 872-878

View : 25 | Download : 10

Publication Date : 2015-12-01

Article Type : Research Paper

Abstract :Acrylamide insert ignore into journalissuearticles values(AA); has neurotoxic, mutagenic, and genotoxic effects in humans and experimental animals. Fruit consumption is important for human health, because fruits are the source of many nutrients such as vitamins, minerals, carotenoids, dietary fiber, and phytonutrients. Many agricultural products provide natural melatonin in the diet. At the onset of the study, rats were weighted and randomly divided into four groups each containing 10 rats as follows: group 1: control insert ignore into journalissuearticles values(fed with normal diet and normal drinking water);; group 2: apricot insert ignore into journalissuearticles values(fed with a daily diet with 5% apricot and normal drinking water);; group 3: AA insert ignore into journalissuearticles values(administered daily acrylamide at 500 μg/kg b.w. Via drinking water and fed a normal diet);; group 4: apricot-AA insert ignore into journalissuearticles values(administered daily acrylamide at 500 μg/kg b.w. Via drinking water and fed with a diet with 5% apricot);. The diet schedule was continued for 12 weeks. At the end of the study, samples of large intestine were collected for biochemical analyses. The highest lipid peroxidation insert ignore into journalissuearticles values(as malondialdehyde, MDA); levels were observed in the AA groups, but MDA levels decreased significantly insert ignore into journalissuearticles values(P < 0.05); with apricot intake. Glutathione peroxidase activity in the apricot-AA group was higher than in the other three groups insert ignore into journalissuearticles values(P < 0.05);. Glutathione S-transferase insert ignore into journalissuearticles values(GST); enzyme activity increased significantly in the AA group as compared with the other groups insert ignore into journalissuearticles values(P < 0.05);. However, GST activity was significantly insert ignore into journalissuearticles values(P < 0.05); decreased by the apricot-supplemented diet. GST-Pi mRNA levels in the AA group increased significantly insert ignore into journalissuearticles values(P < 0.05); as compared with the other groups. In conclusion, the results of the current study demonstrated that AA caused large intestine damage and showed the efficiency of apricot in preventing this damage by inhibiting lipid peroxidation and improving antioxidant enzyme activities.
Keywords : Apricot, acrylamide, rat, large intestine, GST Pi, antioxidant enzymes