Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers

Authors : MAHMOODREZA SHABANIMOFRAD, MOHD RAFII YUSOP, SADEGH ASHKANI, MOHAMED HANAFI MUSA, NUR AZURA ADAM, IFFAH HAIFA, ABDUL RAHIM HARUN, MOHAMMAD ABDUL LATIF

Pages : 666-673

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Publication Date : 2015-12-01

Article Type : Research Paper

Abstract :Abstract: Developing cultivars resistant against different biotypes of brown planthopper insert ignore into journalissuearticles values(BPH); through marker-assisted selection insert ignore into journalissuearticles values(MAS); is an effective management strategy to control this pest. In this study, 28 polymorphic simple sequence repeat insert ignore into journalissuearticles values(SSR); markers were analyzed in 108 F3 progenies derived from the cross of Rathu Heenati and MR276 rice cultivars to investigate the association with BPH resistance against biotypes 2 and 3. For statistical analysis, parents and their offspring were grouped into 2 phenotypic classes based on their levels of BPH resistance. Chi-square analysis demonstrated a good fit to a ratio of 3:1 for the segregation of resistance and susceptibility. Association of SSR markers with phenotypic traits in F3 progenies were identified. Six SSR markers insert ignore into journalissuearticles values(RM401, RM5953, RM217, RM210, RM242, and RM1103); were significantly associated with BPH resistance to biotypes 2 and 3 of BPH. Out of these 6 markers, RM401, RM5953, and RM217 accounted for about 17% of total phenotypic variation and RM210, RM242, and RM1103 accounted for 20% of total phenotypic variation against biotypes 2 and 3, respectively. Therefore, the above 6 markers were confirmed for MAS in rice breeding programs to develop BPH-resistant rice cultivars.
Keywords : Bph resistance gene, F3 progenies, microsatellites, rice