The effect of marT gene on biofilm production of Salmonella Typhimurium

Authors : BURCU YENER İLÇE, NEFİSE AKÇELİK

Pages : 722-731

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Publication Date : 2015-12-01

Article Type : Research Paper

Abstract :Abstract: This study investigates the effect of MarT regulator protein on the production of Salmonella Typhimurium biofilm under in vitro culture conditions. The biofilm formation showed a statistically significant decrease compared to the wild-type strain at the two consecutive concentrations of arabinose insert ignore into journalissuearticles values(0.02% and 0.01%); in which the production of MarT protein was promoted to the highest level. When comparing the biofilm formation capacity to the wild type, the mutants containing a mutation in the genes investigated in terms of marT regulation showed statistically significant reductions, which were identified as fliA insert ignore into journalissuearticles values(59%);, fliC insert ignore into journalissuearticles values(40%);, fimA insert ignore into journalissuearticles values(46%);, fidL insert ignore into journalissuearticles values(25%);, and misL insert ignore into journalissuearticles values(25%); mutants. The biofilm structures of all strains used in this study were detected as the rdar morphotype in agar plates. Among the genes examined, fimA, slsA, fliA, dps, cheA, and wzzB genes were found to have a direct effect on the pellicle formation of S. Typhimurium. Finally, the biofilm formation features of the mutant strain, in which marT expression is induced, on glass, stainless steel, and polystyrene were also examined, and it was determined that the ability to produce biofilm decreased on all surfaces.
Keywords : Biofilm, Salmonella enterica serovar Typhimurium, marT, Red ET recombination system