Improvement of seed germination and in vitro propagation of Bupleurum latissimum Nakai through embryogenesis

Authors : KEE HWA BAE

Pages : 732-739

View : 13 | Download : 10

Publication Date : 2015-12-01

Article Type : Research Paper

Abstract :Abstract: Bupleurum latissimum Nakai is a critically important endangered plant belonging to the family Apiaceae. Seed germination was promoted by soaking in the dormancy breaker gibberellic acid insert ignore into journalissuearticles values(GA3); insert ignore into journalissuearticles values(50% germination as compared with 4% of the control);. An optimal temperature for germination of seed previously soaked in GA3 solution was determined by incubation at various temperatures. Seed germination of 31.8% was observed at 25 °C. These results indicate that the seeds of B. latissimum are difficult to germinate, even when treated with GA3. The greatest callus induction insert ignore into journalissuearticles values(94.8%); was observed in root explants of seedlings grown on MS medium containing a specific concentration of 2,4-dichlorophenoxyacetic acid insert ignore into journalissuearticles values(2,4-D); insert ignore into journalissuearticles values(1.0 mg L-1); and N6-benzyladenine insert ignore into journalissuearticles values(BA); insert ignore into journalissuearticles values(3.0 mg L-1);. Induction of somatic embryo was observed in 78.5% of the root segment cultured on MS medium containing 3.0 mg L-1 2,4-D alone. The highest shoot induction rate was obtained in MS medium containing 30 g L-1 sucrose insert ignore into journalissuearticles values(number of shoots, 6.8; average length of shoot, 8.8 cm);. After acclimation in artificial soil insert ignore into journalissuearticles values(1:1:1 mixture of peat moss, perlite, and sand); and transfer to the greenhouse, 98% of plantlets survived over 2 months. This in vitro propagation protocol will be very useful for the conservation of this critically endangered plant.
Keywords : Bupleurum latissimum Nakai, callus, endangered, endemic, GA3, regeneration