Effects of a Western-style diet high in cholesterol and saturated faton the rabbit exocrine pancreas

Authors : MARÍA ALBA MARTÍNEZ BURGOS, MARIA DOLORES YAGO TORREGROSA, MARIA DOLORES MESA GARCÍA, MARIA BELEN LÃ`PEZ MILLÁN, JESÚS ALCALÁ BEJARANO CARRILLO, ANA RIVAS VELASCO, ÁNGEL GIL HERNÁNDEZ, MARIANO MAÃ`AS ALMENDROS

Pages : 765-774

View : 17 | Download : 13

Publication Date : 2015-12-01

Article Type : Research Paper

Abstract :Abstract: Despite the well-known cardiovascular effects of an atherogenic diet, there are no data relating to its effect on the exocrine pancreas. Therefore, the aim of this study was to investigate the consequences of this eating pattern on the exocrine pancreas. Twelve New Zealand rabbits were randomised to one of two dietary groups, control group insert ignore into journalissuearticles values(C); and hypercholesterolemic insert ignore into journalissuearticles values(HCHOL);, that were fed for 50 days with a standard chow or a diet containing 95.7% standard chow, 3% lard, and 1.3% cholesterol, respectively. Pancreatic enzyme activity, cholesterol, and membrane fatty acids were determined by established methods. The activity of pancreatic lipase insert ignore into journalissuearticles values(6.46 ± 0.948 vs. 1.40 ± 0.460; P < 0.05);, colipase insert ignore into journalissuearticles values(8.93 ± 2.138 vs. 2.36 ± 0.512; P < 0.05);, and chymotrypsin insert ignore into journalissuearticles values(18.54 ± 3.125 vs. 9.69 ± 1.363; P < 0.05); were greater in group HCHOL than in group C. The HCHOL diet increased monounsaturated fatty acids and decreased saturated fatty acids in pancreatic plasma membranes compared with the standard chow. These results suggest a homeostatic adaptation of the pancreas to an atherogenic diet and a greater resistance to the development of lesions than exists in other organs. Further studies are needed, given the lack of research on this issue.
Keywords : Western diet, exocrine pancreas, membrane fatty acids, cholesterol, saturated fat, rabbit