Molecular cytogenetic identification of a novel hexaploid wheat-Thinopyrum intermedium partial amphiploid with high protein content

Authors : Mariyana GEORGIEVA, Klaudia KRUPPA, Nedyalka TYANKOVA, Marta Molnar LANG

Pages : 554-560

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Publication Date : 2016-12-01

Article Type : Research Paper

Abstract :Genomic in situ hybridization and fluorescence in situ hybridization were used to define the cytogenetic constitution of the intergeneric introgression line 29insert ignore into journalissuearticles values(1-57); obtained from the wheat × Thinopyrum intermedium insert ignore into journalissuearticles values(wheatgrass); cross. This line is a high-protein line resistant to leaf rust, yellow rust, and powdery mildew under field conditions and has 42 chromosomes. GISH analysis using a total genomic DNA probe from Thinopyrum intermedium resulted in the identification of 14 chromosomes of wheatgrass origin. The sequential GISH patterns obtained using St-genomic and J-genomic DNA as probes and ABD-genomic DNA from wheat as a blocker led to a better determination of the genomic type of wheatgrass chromosomes. Rearrangements involving Thinopyrum chromosomes and the A, B, and D genomes of wheat were not observed. FISH using the repetitive DNA probes pSc119.2, Afa-family, and pTa71 allowed for identification of all the wheat chromosomes present.
Keywords : FISH, GISH, wheat Thinopyrum intermedium introgression line