Tracking coformulated strains of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae using SCAR markers

Authors : VENKATA RAVI SANKAR UMMIDI, PADMAJA VADLAMANI

Pages : 634-642

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Publication Date : 2016-12-01

Article Type : Research Paper

Abstract :This article examines the development of strain-specific sequence-characterized amplified region insert ignore into journalissuearticles values(SCAR); molecular markers in two strains of Beauveria bassiana and Metarhizium anisopliae, as well as their use for tracking pathogens in coinfected insect pests. The markers were designed based on the polymorphic introns of the large subunit region of the ribosomal DNA. These markers were used to distinguish between two strains of Beauveria insert ignore into journalissuearticles values(B55 and B51); and two strains of Metarhizium insert ignore into journalissuearticles values(M20 and M48);. The entomopathogenic strains demonstrated synergistic increase in mortality against Spodoptera litura insert ignore into journalissuearticles values(Fabricius); insert ignore into journalissuearticles values(Lepidoptera: Noctuidae); larvae when infected with the coformulated M. anisopliae strain M20 + B. bassiana strain B55, particularly at a 2:1 proportion of LC50 concentration. The study revealed a disparity between intergeneric and interstrain coformulations. In intergeneric coformulations, one strain appeared predominant over the other strain at 1:2 and 1:4 proportions, both under in vivo and in vitro conditions. On the other hand, in interstrain coformulations, both strains survived and formed heterokaryons. Molecular studies revealed that the heterokaryons were unstable and reverted back to any one of the parent strains after 3 or 4 generations.
Keywords : Coformulations, sequence characterized amplified region markers, Spodoptera litura, synergistic effect