Identification, characterization, and expression profiling of salt-stress tolerant proton gradient regulator 5 (PGR5) in Gossypium arboreum

Authors : Muhammad SHAHID, Adil JAMAL, Beenish AFTAB, Bahaeldeen Babiker MOHAMED, Javed WATTOO, Muhammad Sarfraz KIANI, Bushra RASHID, Tayyab HUSNAIN

Pages : 889-898

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Publication Date : 2016-12-01

Article Type : Research Paper

Abstract :Salinity is among the significant abiotic stresses adversely affecting plant development and yield for a wide range of crops. For quantitative and qualitative expression studies of proton gradient regulator 5 insert ignore into journalissuearticles values(PGR5); under salt stress, differential display RT-PCR and rapid amplification of cDNA ends insert ignore into journalissuearticles values(RACE); were performed on salt-stress responsive Gossypium arboreum. Alignment of genomic and cDNA sequences revealed that the GPGR5 gene comprises a single open reading frame of 96 amino acids and contains no introns. Alignment of cotton GPGR5 complete amino acid sequence with PGR5 from other plants revealed the following identities: Gossypium raimondii insert ignore into journalissuearticles values(97%);, Amaranthus hybridus insert ignore into journalissuearticles values(72%);, Vitis vinifera insert ignore into journalissuearticles values(69%);, Medicago truncatula insert ignore into journalissuearticles values(68%);, Cucumis melo insert ignore into journalissuearticles values(62%);, Arabidopsis thaliana insert ignore into journalissuearticles values(62%);, Portulaca oleracea insert ignore into journalissuearticles values(61%);, Portulaca grandiflora insert ignore into journalissuearticles values(60%);, and Zea mays insert ignore into journalissuearticles values(55%);. The expression profile was studied in different plant tissues insert ignore into journalissuearticles values(stem, leaf, and root); under the abiotic stresses salt, drought, and cold. The results showed a 7-fold increased expression of GPGR5 in leaf tissue in salt stress and almost no induction of transcription in root and stem tissues in salt stress. This gene has a good expression pattern under cold stress compared to salt and drought. In future studies, GPGR5 may be used as a prognostic marker for the development of abiotic-stress-tolerant plants.
Keywords : Gossypium arboreum, differential display PCR, RACE, proton gradient regulator 5, expression profiling