Human embryonic stem cell N-glycan features relevant to pluripotency

Authors : Sabire KARAÇALI

Pages : 1050-1058

View : 23 | Download : 13

Publication Date : 2016-12-01

Article Type : Research Paper

Abstract :Human embryonic stem cells insert ignore into journalissuearticles values(hESCs); and reprogrammed human induced pluripotent stem cells insert ignore into journalissuearticles values(hiPSCs); proliferate indefinitely in the undifferentiated state insert ignore into journalissuearticles values(self-renewal); and differentiate into the three germ layers insert ignore into journalissuearticles values(and ultimately into all cell types); insert ignore into journalissuearticles values(pluripotency);. Branching in the N-glycan core of hESCs/hiPSCs is limited. hESCs/hiPSCs have high mannose-type and biantennary complex-type core structures, indicating immature stages of N-glycoproteins. The two branches on the core consist of type 2 N-acetyllactosamine insert ignore into journalissuearticles values(LacNAc);, completed mainly with α 2,6-linked sialic acid. Complex terminal fucosylation, particularly α 1,2-linked fucosylation, is another characteristic N-glycosylation feature of hESCs/hiPSCs. All of these structural features are probably associated with the pluripotency of hESCs/hiPSCs. In differentiated cells, two-, three-, and four-branched N-glycan core structures are seen and N-acetylhexosamine compositions increase in the branches. The elongation and size of the branches in the N-glycan cores increase by the addition of poly-LacNAc chains and are the typical features during differentiation. As α 1,2-linked terminal fucosylation disappears, α 1,6-linked core fucosylation increases. It is necessary to know the pluripotency-associated glycans in order to identify and isolate the pluripotent cells from heterogeneous populations containing differentiated cells.
Keywords : Human embryonic stem cell N glycans, N glycan core branching, pluripotency, differentiation