Baculoviral vector loaded mesenchymal stem cells as efficient gene therapy tools for cancer treatment

Authors : SEDEF HANDE AKTAŞ, HAKAN AKBULUT, AYŞE ESER ELÇİN, MAHMUT PARMAKSIZ, ARZU KESKİN AKTAN, ARZU ÇÖLERİ CİHAN, YAŞAR MURAT ELÇİN, FİKRİ İÇLİ

Pages : 1121-1128

View : 17 | Download : 14

Publication Date : 2016-12-01

Article Type : Research Paper

Abstract :Insufficient targeting of the therapeutic genes to tumor cells is one of the major reasons for failure in cancer gene therapy. Mesenchymal stem cells insert ignore into journalissuearticles values(MSCs); seem to be a good candidate as a carrier for gene therapy because of its selective tumor tissue-homing properties. In the current study, we constructed baculoviral vectors insert ignore into journalissuearticles values(BVs); carrying cytosine deaminase insert ignore into journalissuearticles values(CD); insert ignore into journalissuearticles values(BV-CD); or green fluorescence protein insert ignore into journalissuearticles values(GFP); genes insert ignore into journalissuearticles values(BV-GFP); and tested the transduction efficiency of the vectors in tumor and mesenchymal stem cells. We also tested the in vivo efficacy of the BV-CD vector in a colon cancer model. Our results showed that the recombinant baculoviral vectors can efficiently transduce mammalian cells and express genes of interest. The BV-CD vector treatment caused significant in vitro cytotoxicity when used with 5-fluorocytosine. MSCs loaded with the BV-CD vector caused a significant delay in tumor growth and increased survival when compared to control and MSC alone treated groups bearing colon cancer.
Keywords : Our results show that the recombinant BV CD vector could be used either alone or loaded into MSCs in the treatment of established tumors