Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative/antioxidative status

Authors : Milos MATIC, Milica PAUNOVIC, Branka OGNJANOVIC, Andras STAJN, Zorica SAICIC

Pages : 292-301

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Publication Date : 2017-12-01

Article Type : Research Paper

Abstract :Reduced migration capacity of trophoblast cells leads to poor placentation and correlates with severe pregnancy disorders such as intrauterine growth restriction and preeclampsia. Neuropeptide Y insert ignore into journalissuearticles values(NPY); is sympathetic cotransmitter involved in various physiological processes and its levels are significantly increased in preeclamptic pregnancy compared to healthy pregnancy. In this study the prooxidative role of NPY and its effects on migration capacity of human trophoblast cell line JEG-3 were investigated together with the effects of nitric oxide insert ignore into journalissuearticles values(NO); depletion, a molecule that was shown to play an important role in promoting cell migration. The cells were treated for 24 h insert ignore into journalissuearticles values(short-term stimulation); and 72 h insert ignore into journalissuearticles values(long-term stimulation); respectively with 1 nM NPY. Oxidative/antioxidative status and the migration index of cells were measured. The results showed increased concentrations of oxidative stress parameters insert ignore into journalissuearticles values(O2.-, H2O2); and molecules of the antioxidant defense system insert ignore into journalissuearticles values(reduced glutathione and oxidized glutathione);, while the levels of intracellular nitrites insert ignore into journalissuearticles values(indicators of NO); and cell migration index were significantly decreased in trophoblast cells treated with NPY insert ignore into journalissuearticles values(both at 24 h and 72 h of exposure); compared to the control cells. These results suggest that NPY may significantly contribute to reduced migration capacity of trophoblast cells by generating oxidative stress and reducing the bioavailability of NO.
Keywords : Neuropeptide Y, choriocarcinoma trophoblasts, oxidative stress, nitric oxide, migration