Cell-compatible PHB/silk fibroin composite nanofiber mat for tissue engineering applications

Authors : ZEYNEP KARAHALİLOĞLU

Pages : 503-513

View : 20 | Download : 7

Publication Date : 2017-12-01

Article Type : Research Paper

Abstract :Cell compatibility is one of the prominent requirements for the fabrication of tissue engineering materials. Silk fibroin insert ignore into journalissuearticles values(SF); is an excellent material for biomedical applications and shows desirable properties such as good compatibility, minimal tissue reaction, and tailorable degradability. Poly[insert ignore into journalissuearticles values(R);-3-hydroxybutyrate] insert ignore into journalissuearticles values(PHB); has a high percentage of crystallinity and a high melting temperature. In this study, PHB and SF were blended together to improve the mechanical properties of the SF fibrous structure and the crystallinity of PHB. Furthermore, the cell-biomaterial interaction on the PHB/SF composite scaffold was expected to be enhanced via the SF. For this purpose, PHB/SF composite scaffolds were prepared through the use of the electrospinning technique, which is a unique method for the production of biomaterials on the nanoscale intended for tissue engineering. PHB/SF scaffolds were prepared with different SF contents and a suitable electrospinning condition was chosen in terms of structure and fiber diameter. The average fiber diameter was 92 ± 2.6 nm at a flow rate of 0.4 mL/h, distance of 20 cm, polymer concentration of PHB/SF-0 of 7%:18% insert ignore into journalissuearticles values(w/w); or 1:1 insert ignore into journalissuearticles values(v/v);, and voltage of 20 kV. Mechanical and crystalline properties of the PHB/SF scaffold were investigated. Adhesion and proliferation of L929 and HaCaT insert ignore into journalissuearticles values(mouse fibroblast and immortalized human keratinocyte cell lines); on PHB/SF-0 were examined.
Keywords : Poly R, 3 hydroxybutyrate, silk fibroin, tissue engineering, electrospinning, biocompatibility