Regulation of functional activities and essential oil production in Vetiveria zizanioides L. Nash after γ-irradiated sodium alginate elicitation

Authors : ASFIA SHABBIR, MOHAMMAD MASROOR AKHTAR KHAN, YAWAR SADIQ, HASSAN JALEEL, BILAL AHMAD, MOIN UDDIN

Pages : 661-672

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Publication Date : 2017-12-01

Article Type : Research Paper

Abstract :Upon irradiation, marine polysaccharides undergo depolymerization, leading to formation of oligosaccharides that elicit various biological activities in plants. Taking a step further on the previously established growth-promoting activity of irradiated sodium alginate insert ignore into journalissuearticles values(ISA);, structural rearrangements in ISA were analyzed using complementary techniques to develop an understanding of the structure-property relationship. The essential oil insert ignore into journalissuearticles values(EO); of vetiver insert ignore into journalissuearticles values(Vetiveria zizanioides L. Nash); immensely benefits the perfumery industry, proving itself as an economically important crop. A pot experiment was designed to test the effect of water-soluble ISA fractions on the growth, physiology, and EO production of vetiver. The structural characterization of radiation-induced sodium alginate was carried out using SEM, FT-IR, and UV-Vis spectroscopy. Of the various treatments employed, ISA-120 insert ignore into journalissuearticles values(ISA applied at 120 mg L-1); proved the best for most of the parameters studied, including fresh and dry weight of plants and photosynthetic attributes. As compared to the control insert ignore into journalissuearticles values(water-spray treatment);, foliar feeding of ISA-120 resulted in an increase of 21.2% in chlorophyll content, while this treatment enhanced the chlorophyll fluorescence by 21.8% at 300 days after transplanting. Application of ISA-120 also increased the EO content by 21.1% and EO yield by 47.6%. Gas chromatography revealed an increase of 30.1% and 92.6% in the values of content and yield of khusimol, respectively, over the control.
Keywords : Irradiated sodium alginate, Vetiveria zizanioides, FT IR, scanning electron microscopy, essential oil, gas chromatography