Silver nanoparticles induced genotoxicity and oxidative stress in tomato plants

Authors : FAZİLET ÖZLEM ÇEKİÇ, SEFA EKİNCİ, Müslüm Süleyman İNAL, Dilek ÖZAKÇA

Pages : 700-707

View : 18 | Download : 8

Publication Date : 2017-12-01

Article Type : Research Paper

Abstract :Among nanoparticles, silver nanoparticles insert ignore into journalissuearticles values(AgNPs); are intensively used in many materials owing to their antibacterial effects. In the present study different concentrations of AgNPs in Hoagland solution were applied to tomato seedlings. Total chlorophyll content, relative water content insert ignore into journalissuearticles values(RWC);, antioxidant enzyme activities, and malondialdehyde content insert ignore into journalissuearticles values(MDA); as well as the genomic template stability insert ignore into journalissuearticles values(GTS); were analyzed. The intersimple sequence repeat polymerase chain reaction assay insert ignore into journalissuearticles values(ISSR-PCR); was used to determine the genotoxic effects of AgNPs on DNA. RWC did not change under AgNPs treatments; however, total chlorophyll content was significantly reduced by AgNPs applications. ISSR profiles demonstrated a consistent increase in polymorphic bands by the increase in the concentration of AgNPs. GTS value was also reduced depending on the concentration of AgNPs. SOD and APX activities were increased under low AgNPs treatments; however, these activities were decreased under high concentrations of AgNPs treatments. Tomato plants could be sensitive to AgNPs within the increase in MDA content in all of the AgNPs treatments. AgNPs nanotoxicity could be quite dose-dependent. AgNPs could also have negative effects on tomato plants by enhancing DNA damage and lipid peroxidation.
Keywords : AgNP, antioxidant enzymes, ISSR PCR, Solanum lycopersicum L