In vitro evaluation of PLLA/PBS sponges as a promisingbiodegradable scaffold for neural tissue engineering

Authors : İrem Ayşe Kanneci ALTINIŞIK, Fatma Neşe KÖK, Deniz YÜCEL, Gamze KÖSE

Pages : 734-745

View : 19 | Download : 6

Publication Date : 2017-12-01

Article Type : Research Paper

Abstract :In tissue engineering, the use of poly-L-lactic acid insert ignore into journalissuearticles values(PLLA);/polybutylene succinate insert ignore into journalissuearticles values(PBS); blend for the construction of scaffold is very limited. Moreover, polymeric sponges fabricated from PLLA/PBS have not been studied for neural tissue engineering. In the present study, the potential of the utility of PLLA/PBS polymeric sponges seeded with Schwann cells was investigated. PLLA and PBS were blended in order to increase the processability and tune the crystallinity, porosity, and degradation rate of the resulted polymeric sponges. These sponges were then seeded with Schwann cells. Porosity analysis showed that there were no significant differences between different compositions of PLLA/PBS blends; however, the porosity was slightly higher in PLLA/PBS insert ignore into journalissuearticles values(3%, w/v, 2:1); scaffold. Degradation profiles were also investigated for 120 days and almost 25% weight of PLLA/PBS insert ignore into journalissuearticles values(6%, 4%, 2%, w/v, 1:1); scaffolds and 18% weight of PLLA/PBS insert ignore into journalissuearticles values(3%, w/v, 2:1); scaffolds were lost at the end of 120 days. In vitro cell culture studies were also performed and the results proved that all PLLA/PBS blended scaffolds were biocompatible. The highest cell proliferation was observed for PLLA/PBS insert ignore into journalissuearticles values(3%, w/v, 2:1); scaffolds and this construct can be considered a promising biodegradable scaffold for neural tissue engineering.
Keywords : Biomaterials, neural tissue engineering, polybutylene succinate, poly L lactic acid, Schwann cells