Interaction of adipose-derived mesenchymal stem cells with MCF-7 cells in vitro:a study emphasizing signaling molecule expression and transcriptional changes

Authors : Qiong WU, Changxin JIN, Hui CHEN, Xueyong LI, Yuejin LI

Pages : 785-795

View : 18 | Download : 8

Publication Date : 2017-12-01

Article Type : Research Paper

Abstract :Adipose-derived stem cells insert ignore into journalissuearticles values(ADSCs); promote metastasis of breast cancer cells that can differentiate into carcinoma-associated cells in tumor microenvironments. However, the precise mechanism is poorly understood. This study shows that interaction of ADSCs with breast cancer MCF-7 cells changes the level of metastasis-related functional proteins in MCF-7 cells, as well as that of oncogenes in ADSCs. ADSCs were isolated from adipose tissues of patients. The interaction of ADSCs with MCF-7 cells was performed by coculturing ADSCs insert ignore into journalissuearticles values(or MCF-7 cells); with exosomes derived from MCF-7 cells insert ignore into journalissuearticles values(or ADSCs);. Exosomes were labeled by DiI. In cocultures, migration-related regulators in MCF-7 cells were significantly enhanced in both protein insert ignore into journalissuearticles values(Smad, Slug, Snail1/2, Twist1, N-cadherin, vimentin); and mRNA insert ignore into journalissuearticles values(SMAD, SLUG, SNAIL1/2, TWIST1, N-cadherin, and vimentin); levels. The expression levels of oncogenes insert ignore into journalissuearticles values(RAS and HER-2); and an antioncogene gene insert ignore into journalissuearticles values(P53); in ADSCs were upregulated and downregulated, respectively. Interestingly, variation tendencies of the molecules were more conspicuous in inflammatory circumstances than without. In conclusion, interaction of MCF-7 cells with ADSCs increases the levels of a series of metastasis-related functional proteins in MCF-7 cells and enhances the expression of oncogenes in ADSCs.
Keywords : Key words Adipose derived stem cells, metastasis, functional proteins, oncogene