N-Acetylglucoseamine modified alginate sponges as scaffolds for skin tissue engineering

Authors : Murat DEMİRBİLEK, Nelisa TÜRKOĞLU, Selçuk AKTÜRK

Pages : 796-807

View : 20 | Download : 9

Publication Date : 2017-12-01

Article Type : Research Paper

Abstract :In the treatment of dermal wounds, wound-dressing materials prepared from natural mucopolysaccharides are widely used because of their advantages such as nonirritation, nontoxicity, and ease in topical application. In the present study, alginate hydrogels modified with N-acetyl glucose amine insert ignore into journalissuearticles values(NAG); were prepared as wound-dressing material. Physical, chemical, thermal, and mechanical properties of the hydrogels were studied. Cytotoxicity of the hydrogels on endothelial insert ignore into journalissuearticles values(HUVEC); and keratinocyte insert ignore into journalissuearticles values(HaCaT); cells were examined. Anti- and proinflammatory cytokine levels of human monocyte-macrophage cells insert ignore into journalissuearticles values(THP-1); stimulated with hydrogels were determined. According to the results, increasing the NAG concentration led to an increase in the swelling and nitrogen ratios in the hydrogels. Additionally, increasing the NAG concentration decreased elastic modulus and degradation time. Hydrogels were not cytotoxic on HaCaT and HUVEC cells. It stimulated IL-10 and TNF-alpha levels at a small rate.
Keywords : Biomaterials, alginate hydrogels, hyaluronic acid monomers, cytokines