Effects of celecoxib and L-NAME on apoptosis and cell cycle ofMCF-7 CD44+/CD24-/low subpopulation

Authors : Maryam MAJDZADEH, Shima ALIEBRAHIMI, MELODY VATANKHAH, SEYED NASSER OSTAD

Pages : 826-934

View : 13 | Download : 6

Publication Date : 2017-12-01

Article Type : Research Paper

Abstract :Recent studies have reported that cancer stem cells insert ignore into journalissuearticles values(CSCs); play a pivotal role in treatment failure, causing cancer recurrence. Here, we investigated the effects of L-NAME insert ignore into journalissuearticles values(an iNOS inhibitor); and celecoxib insert ignore into journalissuearticles values(a selective COX-2 inhibitor); on CSC-like cells insert ignore into journalissuearticles values(CSC-LCs); and their parental cells. Breast CSC-LCs derived from the MCF-7 cell line were sorted and characterized with the CD44+/CD24-/low phenotype. After isolation, the percentage of the subpopulation expressing CD44+/CD24-/low biomarkers increased considerably from 0.96% to 28.6%. Use of L-NAME and celecoxib showed antiproliferative activity towards both MCF-7 and CSC-LCs. Although celecoxib enhanced apoptotic cell death, the CSC-LC population was more resistant than parental cells. Moreover, L-NAME was less effective at inducing apoptosis, suggesting an involvement of different mechanisms of cell death. L-NAME caused cell cycle arrest in the S-phase in CSC-LCs, while celecoxib induced G0/G1 arrest in CSC-LCs and their parental cells. Immunocytochemistry results demonstrated that L-NAME had a similar potency to attenuate iNOS expression in MCF-7 and CSC-LCs; however, celecoxib reduced COX-2 expression in MCF-7 cells. The results show the crucial role of NOS and COX-2 in the maintenance of CD44+/CD24-/low breast CSC-LCs and suggest that L-NAME and celecoxib could have clinical implication in combination therapy.
Keywords : Celecoxib, L NAME, cancer stem cell like cells, CD44 CD24 low, cytotoxicity