Overexpression of the IbOr gene from sweet potato (Ipomea batatas `Hoang Long`) in maize increases total carotenoid and ß-carotene contents

Authors : Thi Luong TRAN, Thi Huong HO, Duc Thanh NGUYEN

Pages : 1003-1010

View : 14 | Download : 8

Publication Date : 2017-12-01

Article Type : Research Paper

Abstract :Nutritional quality of most maize varieties is very low due to the lack of lysine and tryptophan and extremely low provitamin A carotenoids including ß-carotene, Α-carotene, and ß-cryptoxanthin. In this study, we report the successful overexpression of the IbOr gene in H145 and H95 inbred maize lines under the control of maize seed-specific promoter globulin 1 insert ignore into journalissuearticles values(Glo1); for the purpose of improving ß-carotene in maize. The results showed that the total carotenoid and ß-carotene content of all analyzed transgenic maize plants were significantly higher than those of wild-type lines. For H145-IbOr transgenic maize, in the best line insert ignore into journalissuearticles values(H145-IbOr.10);, the total carotenoid and ß-carotene contents were increased up to 10.36- and 15.11-fold, respectively, compared to the wild type insert ignore into journalissuearticles values(H145-WT);. In the case of H95-IbOr transgenic plants, 5.58-fold increase in total carotenoid and 7.63-fold increase in ß-carotene were achieved in the H95-IbOr.6 line compared to nontransgenic plants insert ignore into journalissuearticles values(H95-WT);. In all the transgenic plants derived from the wild-type maize line with less carotenoid content insert ignore into journalissuearticles values(H145-WT);, the content of both total carotenoid and ß-carotene was higher than in transgenic plants derived from the wild-type maize line having more carotenoid content insert ignore into journalissuearticles values(H95-WT);. Our research is the first in successful overexpression of IbOr gene in maize.
Keywords : ß Carotene, carotenoid content, IbOr gene, maize, sweet potato