Recombinant outer membrane protein Q and putative lipoprotein from Bordetella pertussis inducing strong humoral response were not protective alone in the murine lung colonization model

Authors : Çiğdem YILMAZ, Erkan ÖZCENGİZ, Gülay ÖZCENGİZ

Pages : 123-131

View : 17 | Download : 12

Publication Date : 2018-12-01

Article Type : Research Paper

Abstract :Despite high vaccination coverage after introduction of whole cell insert ignore into journalissuearticles values(wP); and acellular pertussis insert ignore into journalissuearticles values(aP); vaccines, pertussis resurgence has been reported in many countries. aP vaccines are commonly preferred due to side effects of wP vaccines and formulated with aluminum hydroxide insert ignore into journalissuearticles values(Alum);, which is not an effective adjuvant to eliminate Bordetella pertussis . Low efficiency of current aP vaccines is thought to be the main reason for the resurgence for which newer generation aP vaccines are needed. In the present study, immunogenicity and protective efficacy of outer membrane protein Q insert ignore into journalissuearticles values(OmpQ); and a putative lipoprotein insert ignore into journalissuearticles values(Lpp); from B. pertussis were investigated in mice by using two different adjuvants, monophosphoryl lipid A insert ignore into journalissuearticles values(MPLA); or Alum. OmpQ and putative Lpp were cloned, expressed, and purified from Escherichia coli . The proteins were formulated to immunize mice. Both recombinant OmpQ and putative Lpp induced a significant increase in immunoglobulin G1 insert ignore into journalissuearticles values(IgG1); and immunoglobulin G2a insert ignore into journalissuearticles values(IgG2a); responses compared to the control group. Moreover, MPLA-adjuvanted formulations resulted in higher IgG2a levels than Alum-adjuvanted ones. However, there were no significant differences between test and control groups regarding interferon-gamma insert ignore into journalissuearticles values(IFN-γ); levels, and the mice lung colonization experiments indicated that neither rOmpQ nor rLpp could confer protection alone against B . pertussis challenge.
Keywords : Adjuvants, Bordetella pertussis, pertussis vaccines, recombinant proteins