Polyphenolic contents of Teucrium polium L. and Teucrium scordium L. associated with their protective effects against MMC-induced chromosomal damage in cultured human peripheral blood lymphocytes

Authors : Olivera MILOŠEVIĆDJORDJEVIĆ, Marina ADOVIĆ JAKOVLJEVIĆ, Aleksandra MARKOVIĆ, Milan STANKOVIĆ, Andrija ĆIRIĆ, Dragoslav MARINKOVIĆ, Darko GRUJIČIĆ

Pages : 152-162

View : 12 | Download : 6

Publication Date : 2018-12-01

Article Type : Research Paper

Abstract :Teucrium species have been used in traditional medicine for treatment of different diseases. The aim of this study was to investigate polyphenolic contents by high-performance liquid chromatography insert ignore into journalissuearticles values(HPLC);, and the genotoxic effect of methanolic extracts of Teucrium polium and Teucrium scordium using the cytokinesis-block micronucleus insert ignore into journalissuearticles values(CBMN); assay on human peripheral blood lymphocytes insert ignore into journalissuearticles values(PBLs); from healthy donors. The HPLC analysis showed that extracts consist of phenolic acid insert ignore into journalissuearticles values(gallic, vanillic, caffeic, chlorogenic, p-coumaric, sinapic); and flavonoids insert ignore into journalissuearticles values(catechin, rutin, myricetin, luteolin, quercetin and apigenin);. Cultures were treated with extracts of both plants separately and in combinations with mitomycin C insert ignore into journalissuearticles values(MMC);. In separate treatments, both herbal extracts significantly induced micronucleus insert ignore into journalissuearticles values(MN); frequency only at the highest concentrations. All concentrations of T. scordium , except the lowest, and all concentrations of T. polium extracts in combined treatment with MMC significantly reduced the frequency of MN. The extract of T. polium did not significantly affect the nuclear division index insert ignore into journalissuearticles values(NDI);, whereas T. scordium in higher concentrations, separately and in combined treatment with MMC, significantly decreased the NDI value. Our results suggest that both herbal extracts in combination with MMC have antimutagenic insert ignore into journalissuearticles values( T. polium ); and proapoptotic effects insert ignore into journalissuearticles values( T. scordium );, which indicates their protective effects in PBLs.
Keywords : Genotoxic potential, HPLC analysis, Teucrium polium, Teucrium scordium, micronuclei, human peripheral blood lymphocytes