Expression of a bacterial aroA gene confers tolerance to glyphosate in tobacco plants

Authors : Feng LIU, Yueping CAO

Pages : 187-194

View : 15 | Download : 7

Publication Date : 2018-12-01

Article Type : Research Paper

Abstract :Glyphosate is a widely used herbicide that inhibits the 5-enolpyruvylshikimate-3-phosphate synthase insert ignore into journalissuearticles values(EPSPS);-encoding aroA gene in the shikimate pathway. The discovery and cloning of the aroA gene with high resistance is central to breeding a transgenic glyphosate-resistant plant. A novel aroA Pantoea gene from Pantoea G-1 was previously isolated and cloned. The aroA Pantoea enzyme was defined as a new class I EPSPS with glyphosate resistance. The aroA Pantoea gene was introduced into tobacco through Agrobacterium -mediated transformation. The transgenic tobacco plants were confirmed by PCR, RT-PCR, and Southern blot. The analysis of glyphosate resistance also showed that the transgenic tobacco plants could survive at 15 mM glyphosate; the glyphosate resistance level of the transgenic plants is higher than the agricultural application level recommended by most manufacturers. Overall, this study shows that aroA Pantoea can be used as a candidate gene for the development of genetically modified crops.
Keywords : aroA, glyphosate, Pantoea, tobacco, transformation