Increased elastic modulus of plasma polymer coatings reinforced with detonation nanodiamond particles improves osteogenic differentiation of mesenchymal stem cells

Authors : Milena KEREMIDARSKAMARKOVA, Ekaterina RADEVA, Dimitar MITEV, Aleš IGLIČ, Brett PAULL, Pavel NESTERENKO, Joseph ŠEPITKA, İta JUNKAR, Natalia KRASTEVA

Pages : 195-203

View : 15 | Download : 8

Publication Date : 2018-12-01

Article Type : Research Paper

Abstract :  In the present study we demonstrated that composite PPHMDS/DND coatings with elastic moduli close to those of mature bone tissue insert ignore into journalissuearticles values(0.2-2.8 GPa); stimulated growth and osteogenic differentiation of human adipose-derived mesenchymal stem cells insert ignore into journalissuearticles values(hAD- MSCs);. Composite coatings were prepared by a method of plasma polymerization insert ignore into journalissuearticles values(PP); where detonation nanodiamond insert ignore into journalissuearticles values(DND); particles in different amounts insert ignore into journalissuearticles values(0.1, 0.5, and 1 mg/mL); were added to hexamethyldisiloxane insert ignore into journalissuearticles values(HMDS); before plasma deposition. This method allows variation only in the reduced elastic modulus insert ignore into journalissuearticles values(Er`); with increase in the particle concentration, while the other surface properties, including surface wettability and topography, did not change. The response of hAD-MSCs to the increasing stiffness showed an effect on adhesion and osteogenic differentiation but not on cell proliferation. Matrix mineralization and cell spreading were maximized on PPHMDS/DND coatings with the highest elastic modulus insert ignore into journalissuearticles values(2.826 GPa);, while the differences in proliferation rates among the samples were negligible. In general, PPHMDS/DND coatings provide better conditions for growth and osteogenic differentiation of hAD-MSCs in comparison to glass coverslips, confirming their suitability for osteo-integration applications. Additionally, our findings support the hypothesis that biomaterials with elasticity similar to that of the native tissue can improve the differentiation potential of mesenchymal stem cells.
Keywords : Detonation nanodiamonds, organosilicone, bone implants, stiffness, cell adhesion and growth