Evaluation of antiproliferative and protective effects of Eupatorium cannabinum L. extracts

Authors : Alice GRIGORE, Georgeta NEAGU, Nicoleta DOBRE, Adrian ALBULESCU, Lucian IONITA, Carmen IONITA, Radu ALBULESCU

Pages : 341-351

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Publication Date : 2018-12-01

Article Type : Research Paper

Abstract :Eupatorium cannabinum L. insert ignore into journalissuearticles values(Asteraceae); has been used for a long time for medicinal purposes due to its various pharmacological effects and richness in active compounds such as phenolics, sesquiterpenes, pyrrolizidine alkaloids, and polysaccharides. Despite the high content of compounds that have important roles in medicinal plants, there are still limited literature data regarding this valuable species. The plant was fractioned using chloroform insert ignore into journalissuearticles values(EC); and distilled water insert ignore into journalissuearticles values(EA); and HPLC analysis revealed the presence of eupatorin, eupatilin, and quercetin in EC and caffeic acid and rutin in EA. The antiproliferative potential on BT-20, HepG2, Caco-2, and Jurkat cancer cell lines was assessed by MTS test. Jurkat cells were more sensitive to both extracts insert ignore into journalissuearticles values(IC 50 of 7.35 ± 0.35 for EC and 13.77 ± 2.16 ?g/mL for EA);, while the other lines were susceptible only to EC insert ignore into journalissuearticles values(IC 50 88.27 ± 1.34 on Caco-2 cells and over 100 ?g/mL on BT- 20 and HepG2 cells); after 24 h of exposure. In an LPS-induced damage mouse model of endotoxemia, we showed that preventive administration increases the survival times of mice and leads to inhibition of proinflammatory cytokines. Both polar and nonpolar compounds are involved in exerting these effects, but further analytical studies are needed to identify the key responsible compounds and their biochemical pathways.
Keywords : Hemp agrimony, antiinflammatory, methoxyflavones, endotoxemia