Proteomic analysis of heterotic seed germination in maize using F1 hybrid DHM 117 and its parental inbreds

Authors : Rajesh Kumar MEENA, Durgeshwar PULLAIAHGARI, Padmaja GUDIPALLI

Pages : 352-370

View : 23 | Download : 10

Publication Date : 2018-12-01

Article Type : Research Paper

Abstract :  A number of differentially expressed proteins insert ignore into journalissuearticles values(DEPs); were identified in comparative two-dimensional gel electrophoresis analysis of dry and 24-h water-imbibed seeds of maize F1 hybrid DHM 117 insert ignore into journalissuearticles values(BML 6 x BML 7); and its parental inbreds. Of the DEPs, 53.4% insert ignore into journalissuearticles values(86/161); in dry seeds and 58% insert ignore into journalissuearticles values(127/219); in water-imbibed seeds exhibited a nonadditive pattern in the F 1 hybrid as compared to parental inbreds. A total of 30 DEPs were categorized into different biological processes, most of which were related to metabolism and energy insert ignore into journalissuearticles values(34%);, followed by storage proteins insert ignore into journalissuearticles values(27%);, stress response insert ignore into journalissuearticles values(23%);, transcription and translation insert ignore into journalissuearticles values(7%);, cell cycle insert ignore into journalissuearticles values(3%);, and hormone biosynthesis insert ignore into journalissuearticles values(3%);. The transcript accumulation pattern of 8 selected genes corresponding to DEPs was examined using qRTPCR. Interestingly, LEA protein Rab28 showed higher accumulation in dry seeds at both protein and transcript levels, whereas indole- 3-acetaldehyde oxidase showed lower accumulation in water-imbibed seeds of the F1 hybrid than the female parent at the protein level. Thus, the DEPs particularly involved in metabolic and energy processes, as well as hormone biosynthesis in the F 1 hybrid, might be responsible for heterotic seed germination in the F 1 hybrid. The DEPs identified in this study provide a scope for improving the seed germination trait of agricultural crops.
Keywords : Heterosis, two dimensional gel electrophoresis, differentially expressed proteins, F 1 hybrid, seed germination