Identification of bioactive compounds from Fraxinus angustifolia extracts with anti-NADH oxidase activity of bovine milk xanthine oxidoreductase

Authors : Nadjia AHMANE, Dina ATMANIKILANI, Nassima CHAHER, Karima AYOUNI, Meriem RAHMANIBERBOUCHA, Gregory Da COSTA, Nadjet DEBBACHEBENAIDA, Tristan RICHARD, Djebbar ATMANI

Pages : 133-147

View : 19 | Download : 8

Publication Date : 2019-12-01

Article Type : Research Paper

Abstract :Fraxinus angustifolia leaves and bark are used in traditional medicine against various inflammatory-related pathologies incumbent to reactive oxygen species insert ignore into journalissuearticles values(ROS); generation by the NADH oxidase activity of enzymes such as xanthine oxidoreductase insert ignore into journalissuearticles values(XOR);. This study was designed to investigate the in vitro and in vivo inhibitory activities of this enzyme by Fraxinus angustifolia extracts. The leaf organic phase of ethyl acetate insert ignore into journalissuearticles values(LFA); and its bark aqueous counterpart insert ignore into journalissuearticles values(BFA); showed the strongest anti-NADH oxidase activity in vitro insert ignore into journalissuearticles values(IC50 = 38.51 and 42.04 μg mL-1, respectively);. They consequently suppressed superoxide generation both enzymatically insert ignore into journalissuearticles values(53% and 19%, respectively); and nonenzymatically insert ignore into journalissuearticles values(34% and 19%, respectively);. These results were corroborated in vivo, with high anti- NADH oxidase potential of the leaves and bark extracts insert ignore into journalissuearticles values(75.32% and 51.32%, respectively); concomitant with moderate hypouricemic activities insert ignore into journalissuearticles values(36.84% and 38.59%, respectively);. Bio-guided fractionation led to the identification, by LC-DAD-MS/MS, of esculin and calcelarioside in bark and kaempferol glucoside in leaves as the main compounds responsible for the anti-NADH oxidase activity of XOR. These results plead in favor of the use of F. angustifolia as a source of potentially interesting therapeutic substances.
Keywords : Fraxinus angustifolia, NADH oxidase, xanthine oxidoreductase, phenolics, hyperuricemia