Molecular and in silico cloning, identification, and preharvest period expression analysis of a putative cytochrome P450 monooxygenase gene from Camellia sinensis (L.) Kuntze (tea)

Authors : Ayşenur EMİNOĞLU, YEŞİM AKTÜRK DİZMAN, Şule GÜZEL, Ali Osman BELDÜZ

Pages : 1-11

View : 21 | Download : 9

Publication Date : 2018-12-01

Article Type : Research Paper

Abstract :Cytochrome P450 monooxygenases are one of the largest heme-containing protein groups, and the majority of them catalyze hydroxylation reactions dependent on nicotinamide adenine dinucleotide phosphate and oxygen. Cytochrome P450 insert ignore into journalissuearticles values(CYP); enzymes function in a wide range of monooxygenation reactions essential in primary and secondary metabolism in plants. Camellia sinensis insert ignore into journalissuearticles values(L.); Kuntze is a commercially and economically valuable plant due to its medicinally important secondary metabolites and as a beloved beverage. Cytochrome P450 monooxygenases play a significant role in the biosynthesis of a variety of secondary metabolites in tea. Although the biosynthesis of secondary metabolites has been investigated in detail, there have been limited studies conducted on identifying the genetic mechanisms of CYP-catalyzed secondary metabolic pathways in the C. sinensis insert ignore into journalissuearticles values(tea); plant. In our study, we characterized a putative C. sinensis insert ignore into journalissuearticles values(L.); Kuntze cytochrome P450 monooxygenase gene insert ignore into journalissuearticles values(Csp450);, which has 1759 bp full-length cDNA with 49 bp of 5$^{`}$ and 183 bp of 3$^{`}$ untranslated regions. The CDS of the gene is 1527 bp and 508 amino acids in length. BLAST results of the deduced amino acid sequence revealed a high similarity with the CYP704C1-like superfamily. Preharvest period gene expression analysis from May, July, and September did not show any difference.
Keywords : Camellia sinensis L, Kuntze tea, cytochrome P450 monooxygenase, molecular and in silico cloning, seasonal gene expression