Ultrasound-enhanced gene delivery to alfalfa cells by hPAMAM dendrimer nanoparticles

Authors : AMIN AMANI, Nasser ZARE, ASADOLLAH ASADI, Rasool Asghari ZAKARIA

Pages : 63-75

View : 15 | Download : 7

Publication Date : 2018-12-01

Article Type : Research Paper

Abstract :Cationic polyamidoamine insert ignore into journalissuearticles values(PAMAM); dendrimers are highly branched nanoparticles with unique molecular properties, which make them promising nanocarriers for gene delivery into cells. This research evaluated the ability of hyperbranched PAMAM insert ignore into journalissuearticles values(hPAMAM);-G2 with a diethylenetriamine core to interact with DNA, its protection from ultrasonic damage, and delivery to alfalfa cells. Additionally, the effects of ultrasound on the efficacy of hPAMAM-G2 for the delivery and expression of the gusA gene in the alfalfa cells were investigated. The electrophoresis retardation of plasmid DNA occurred at an N/P ratio insert ignore into journalissuearticles values(where N is the number of hPAMAM nitrogen atoms and P is the number of DNA phosphorus atoms); of 3 and above, and hPAMAM-G2 dendrimers completely immobilized the DNA at an N/P ratio of 4. The analysis of the DNA dissociated from the dendriplexes revealed a partial protection of the DNA from ultrasound damage at N/P ratios lower than 2, and with increasing N/P ratios, the DNA was better protected. Sonication of the alfalfa cells in the presence of ssDNA-FITC-hPAMAM increased the ssDNA delivery efficiency to 36%, which was significantly higher than that of ssDNA-FITC-hPAMAM without sonication. Additionally, the efficiency of transfection and the expression of the gusA gene were dependent on the N/P ratio and the highest efficiency insert ignore into journalissuearticles values(1.4%); was achieved at an N/P ratio of 10. The combination of 120 s of ultrasound and hPAMAM-DNA increased the gusA gene transfection and expression to 3.86%.
Keywords : Gene transfer, hPAMAM DNA complex, Medicago sativa L, polyamidoamine dendrimers, sonication