A new Helicoverpa armigera Nucleopolyhedrovirus isolate from Heliothis peltigera (Denis & Schiffermuller) (Lepidoptera: Noctuidae) in Turkey

Authors : Gözde Büşra EROĞLU, Remziye NALÇACIOĞLU, Zihni DEMİRBAĞ

Pages : 340-348

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Publication Date : 2019-12-01

Article Type : Research Paper

Abstract :This study reports a new Helicoverpa armigera nucleopolyhedrovirus insert ignore into journalissuearticles values(NPV); isolated from Heliothis peltigera insert ignore into journalissuearticles values(Denis & Schiffermuller);, collected in the vicinity of Adana, Turkey. Infection was confirmed by tissue polymerase chain reaction and sequence analysis. Results showed that dead H. peltigera larvae contain Helicoverpa armigera nucleopolyhedrovirus. Thus, the isolate was named as HearNPV-TR. Microscopy studies indicated that occlusion bodies were 0.73 to 1.66 μm in diameter. The nucleocapsids are approximately 184 × 41 nm in size. The genome of HearNPV-TR was digested with KpnI and XhoI enzymes and calculated as 130.5 kb. Phylogenetic analysis showed that HearNPV-TR has close relation with the H. armigera SNPV-1073 China isolate. The Kimura analysis confirmed that the isolate is a variant of H. peltigera NPV. Bioassays were performed using six different concentrations insert ignore into journalissuearticles values(1 × 103 to 1 × 108 occlusion bodies insert ignore into journalissuearticles values(OBs);/mL); on 2nd instar larvae of H. peltigera, H. armigera, Heliothis viriplaca, Heliothis nubigera. LC50 values were calculated to be 9.5 × 103, 1.9 × 104, 8.6 × 104 and 9.2 × 104 OBs/mL within 14 days, respectively. Results showed that it is a promising biocontrol agent against Heliothinae species.
Keywords : Safflower, baculovirus, nucleopolyhedrovirus, biocontrol, Heliothis peltigera