Evaluation of recombinant human vascular endothelial growth factor VEGF121-loaded poly-l-lactide microparticles as a controlled release delivery system

Authors : Sunil ABRAHAM, Srinivasa Prasad RANGASWAMY, Amutha CHINNAIAH

Pages : 34-47

View : 12 | Download : 8

Publication Date : 2020-12-01

Article Type : Research Paper

Abstract :Vascular endothelial growth factor A insert ignore into journalissuearticles values(VEGF-A); is an important growth factor that plays a major role in angiogenesis. With different isoforms distributed in various tissues, the shortest isoform of VEGF-A is VEGF121, one of the physiologically functional variants next to VEGF165. It is well known that VEGF has a shorter half-life, and the stability of the protein must be considered in therapeutic aspects. Poly-l-lactide insert ignore into journalissuearticles values(PLA); microparticles can release the encapsulated protein in a sustained release mode. In this study, the VEGF121 gene was cloned and expressed in a prokaryotic expression system insert ignore into journalissuearticles values(Escherichia coli);. The recombinant VEGF121 was encapsulated with PLA microparticles and studied in vitro and ex ovo for the sustained release mechanism. The PLA-VEGF microparticles and the recombinant VEGF121 were explored for their bioactivity in human umbilical vein endothelial cells insert ignore into journalissuearticles values(HUVEC);. VEGF released in vitro from PLA microparticles on days 1, 20, and 30 showed remarkable biological activity compared to PBS-loaded PLA microparticles such as the ability of the cells to proliferate, migrate, and form tubes similar to recombinant VEGF121. Besides, PLA-VEGF microparticles and the recombinant VEGF121 were also tested for their proangiogenic action in embryonated eggs by chicken chorioallantoic membrane assay insert ignore into journalissuearticles values(CAM);, and the effect was observed in both forms. This study suggests that PLA-loaded VEGF microparticles in a sustainable release format can be effectively used in proangiogenic therapy and reduce the adverse effects caused due to multiple dosages.
Keywords : VEGF, purification, poly l lactide, human umbilical vein endothelial cells, proliferation, cell migration, CAM assay, sustained release