Interaction of influenza A virus NS2/NEP protein with the amino-terminal part of Nup214

Authors : Burçak Şenbaş AKYAZI, Ayşegül PİRİNÇAL, Atsushı KAWAGUCHI, Kyosuke NAGATA, Kadir TURAN

Pages : 82-92

Doi:10.3906/biy-1909-49

View : 17 | Download : 7

Publication Date : 2020-12-01

Article Type : Research Paper

Abstract :Influenza A viruses have a single-stranded RNA genome consisting of 8 segments. Each RNA segment associates with the nucleoprotein NP and viral RNA polymerase to and from a viral ribonucleoprotein vRNP particle. The viral mRNA synthesis is dependent on a capped primer derived from nascent host RNA transcripts. For these processes to take place, vRNPs must pass through the cell nuclear pore complex NPC to the nucleus. The influenza A virus NS2 protein, also called the nuclear export protein NES , has an important role in the nucleocytoplasmic transport of vRNPs. This protein interacts with the host cellular nucleoporins during the nuclear export of vRNPs. In this study, the human nucleoporin 214 Nup214 was identified as an NS2-binding protein by using a yeast two-hybrid assay. The interaction between NS2 and human Nup214 was confirmed in both yeast and mammalian cells. It has been shown that the NS2 protein interacts with the amino terminal FG domain of the Nup214 protein. The influenza viral replication was suppressed in knockdown cells for the Nup214 protein. It was concluded that the FG domains of nucleoporins have an important role in the interaction of the influenza NS2 protein with host NPC for vRNA export.
Keywords : Influenza A viruses, NS2 NEP, Nup214, nuclear pore complex, nuclear export