Helicobacter-stimulated IL-10-producing B cells suppress differentiation of lipopolysaccharide/Helicobacter felis-activated stimulatory dendritic cells

Authors : Dogus ALTUNOZ, Ayça SAYI YAZGAN

Pages : 214-224

View : 14 | Download : 7

Publication Date : 2021-12-01

Article Type : Research Paper

Abstract :Regulatory B cells insert ignore into journalissuearticles values(Bregs); produce antiinflammatory cytokines and inhibits proinflammatory response. Recently, immunosuppressive roles of Bregs in the effector functions of dendritic cells insert ignore into journalissuearticles values(DCs); were demonstrated. However, cross talk between Bregs and DCs in Helicobacter infection remains unknown. Here, we showed that direct stimulation of bone marrow-derived DCs insert ignore into journalissuearticles values(BMDCs); with Helicobacter felis insert ignore into journalissuearticles values(H. felis); antigen upregulates their CD86 surface expression and causes the production of interleukin-6 insert ignore into journalissuearticles values(IL-6);, tumor necrosis factor alpha insert ignore into journalissuearticles values(TNF-α);, interleukin-12 insert ignore into journalissuearticles values(IL-12);, and interleukin-10 insert ignore into journalissuearticles values(IL-10);. Furthermore, prestimulation of DCs with supernatants derived from both Helicobacter-stimulated IL-10– B insert ignore into journalissuearticles values(Hfstim-IL-10– B); or IL-10+ B insert ignore into journalissuearticles values(Hfstim-IL-10+); cells suppresses the secretion of TNF-α and IL-6, but does not affect the expression of CD86 and secretion of IL-12 by lipopolysaccharide insert ignore into journalissuearticles values(LPS); or H. felisactivated BM-DCs. Remarkably, soluble factors secreted by Hfstim-IL-10– B cells, but not by Hfstim-IL-10+ B cells, suppress the secretion of IL-10 by BM-DCs upon subsequent LPS stimulation. In contrast, prestimulation with BM-DCs with supernatants of Hfstim-IL-10+ B cells before H. felis antigen stimulation induces significantly their IL-10 production. Collectively, our data indicated that prestimulation with soluble factors secreted by Hfstim-IL-10+ B cells, DCs exhibit a tolerogenic phenotype in response to LPS or Helicobacter antigen by secreting high levels of IL-10, but decreased levels of IL-6 and TNF-α.
Keywords : Helicobacter felis, regulatory B cells, dendritic cells, lipopolysaccharide