IL-1R1 deficiency impairs liver regeneration after 2/3 partial hepatectomy in aged mice

Authors : Deming Lİ, Ze WANG, Chunyan ZHANG, Cunshuan XU

Pages : 225-234

View : 15 | Download : 9

Publication Date : 2021-12-01

Article Type : Research Paper

Abstract :Inflammation has a dual effect: it can protect the body and destroy tissue and cell as well. The purpose of this experiment was to determine the role of IL-1R1 in liver regeneration insert ignore into journalissuearticles values(LR); after partial hepatectomy insert ignore into journalissuearticles values(PH); in aged mice. The wild-type insert ignore into journalissuearticles values(WT, n = 36); and the IL-1R1 knockout insert ignore into journalissuearticles values(KO, n = 36); 24-month-old C57BL/6J mice underwent two-thirds PH; 33 WT mice underwent sham operation. Liver coefficient was calculated by liver/body weight. The mRNA and protein expressions of genes were evaluated by quantitative real-time polymerase chain reaction insert ignore into journalissuearticles values(qRT-PCR); and Western blotting methods, respectively. Compared with WT mice, liver coefficient was lower in the IL-1R1 KO aged mice at 168 and 192 h insert ignore into journalissuearticles values(p = 0.039 and p = 0.027);. The mRNA transcription of inflammation-related genes and cell cycle-associated genes decreased or delayed. The protein expressions of proliferation-related marker PCNA and proliferation-associated signaling pathway components JNK1, NF-kappa B and STAT3 reduced or retarded. There was stronger activation of proapoptotic proteins caspase-3, caspase-8 and BAX in the IL-1R1 KO mice at different time points insert ignore into journalissuearticles values(p < 0.05 or p < 0.01);. IL-1R1 KO reduced inflammation and caused impaired liver regeneration after 2/3 partial hepatectomy in aged mice. Maintaining proper inflammation may contribute to regeneration after liver partly surgical resection in the elderly.
Keywords : Aged mice, IL 1R1, inflammation, liver resection, regeneration