PtdIns(4,5)P2 and PtdIns(3,4,5)P3 dynamics during focal adhesions assembly and disassembly in a cancer cell line

Authors : Dhurgham ALFAHAD, Salem ALHARETHİ, Bandar ALHARBİ, Khatab MAWLOOD, Philip DASH

Pages : 381-392

View : 21 | Download : 7

Publication Date : 2020-12-01

Article Type : Research Paper

Abstract :Focal adhesions insert ignore into journalissuearticles values(FAs); are large assemblies of proteins that mediate intracellular signals between the cytoskeleton and the extracellular matrix insert ignore into journalissuearticles values(ECM);. The turnover of FA proteins plays a critical regulatory role in cancer cell migration. Plasma membrane lipids locally generated or broken down by different inositide kinases and phosphatase enzymes to activate and recruit proteins to specific regions in the plasma membrane. Presently, little attention has been given to the use of phosphatidylinositol 4,5-bisphosphate insert ignore into journalissuearticles values(PtdInsinsert ignore into journalissuearticles values(4,5);P2); and Phosphatidylinositol 3,4,5-trisphosphate insert ignore into journalissuearticles values(PtdInsinsert ignore into journalissuearticles values(3,4,5);P3); fluorescent biosensors in order to determine the spatiotemporal organisation of PtdInsinsert ignore into journalissuearticles values(4,5);P2 and PtdInsinsert ignore into journalissuearticles values(3,4,5);P3 within and around or during assembly and disassembly of FAs. In this study, specific biosensors were used to detect PtdInsinsert ignore into journalissuearticles values(4,5);P2, PtdInsinsert ignore into journalissuearticles values(3,4,5);P3, and FAs proteins conjugated to RFP/GFP in order to monitor changes of PtdInsinsert ignore into journalissuearticles values(4,5);P2 and PtdInsinsert ignore into journalissuearticles values(3,4,5);P3 levels within FAs. We demonstrated that the localisation of PtdInsinsert ignore into journalissuearticles values(4,5);P2 and PtdInsinsert ignore into journalissuearticles values(3,4,5);P3 were moderately correlated with that of FA proteins. Furthermore, we demonstrate that local levels of PtdInsinsert ignore into journalissuearticles values(4,5); P2 increased within FA assembly and declined within FA disassembly. However, PtdInsinsert ignore into journalissuearticles values(3,4,5);P3 levels remained constant within FAs assembly and disassembly. In conclusion, this study shows that PtdInsinsert ignore into journalissuearticles values(4,5);P2 and PtdInsinsert ignore into journalissuearticles values(3,4,5);P3 localised in FAs may be regulated differently during FA assembly and disassembly.
Keywords : Focal adhesions turnover, PtdIns 4, 5, P2, PtdIns 3, 4, 5, P3, Focal adhesions turnover, PtdIns 4, 5, P2, PtdInsinsert ignor