Coculture model of blood-brain barrier on electrospun nanofibers

Authors : Mustafa Gorkem OZYURT, Ece BAYİR, Sule DOGAN, Sukru OZTURK, Aylin SENDEMİR

Pages : 121-132

View : 20 | Download : 8

Publication Date : 2020-12-01

Article Type : Research Paper

Abstract :The blood–brain barrier insert ignore into journalissuearticles values(BBB); is a control mechanism that limits the diffusion of many substances to the central nervous system insert ignore into journalissuearticles values(CNS);. In this study, we designed an in-vitro 3-dimensional BBB system to obtain a fast and reliable model to mimic drug delivery characteristics of the CNS. A support membrane of polycaprolactone nanofiber surfaces was prepared using electrospinning. After confirming the fiber morphology and size, endothelial cells insert ignore into journalissuearticles values(HUVEC); and glial cells were cultured on either side of this membrane. The model’s similarity to in vivo physiology was tested with a home-designed transmembrane resistance insert ignore into journalissuearticles values(TR); device, with positive and negative control molecules. Finally, 2 doses of methotrexate insert ignore into journalissuearticles values(MTX);, a chemotherapy agent, were applied to the model, and its permeability through the model was determined indirectly by a vitality test on the MCF-7 cell line. Nicotine, the positive control, completed its penetration through the model almost instantly, while albumin, the negative control, was blocked significantly even after 2 days. MTX reached a deadly threshold 24 h after application. The TR value of the model was promising, being around 260 ohm.cm2. The provided model proposes a disposable and reliable tool for investigating drug permeability through the BBB and has the potential to reduce the number of animal experiments.
Keywords : Bloodbrain barrier, electrospinning, methotrexate, 3 dimensional cell culture, transmembrane resistance