Poly (I:C)- and doxorubicin-loaded magnetic dendrimeric nanoparticles affect the apoptosis-related gene expressions in MCF-7 cells

Authors : Rouhollah KHODADUST, Aktan ALPSOY, Gozde UNSOY, Ufuk GUNDUZ

Pages : 133-144

View : 16 | Download : 9

Publication Date : 2020-12-01

Article Type : Research Paper

Abstract :Use of nanoparticles as drug carrier vectors has great potential to circumvent the limitations associated with chemotherapy, including drug resistance and destructive side effects. For this purpose, magnetic generation 4 dendrimeric nanoparticles were prepared to carry chemotherapeutic agent doxorubicin insert ignore into journalissuearticles values(G 4-DOX); and immune modulator polyinosinic:polycytidylic acid [Polyinsert ignore into journalissuearticles values(I:C);]. As previously reported, DOX and Polyinsert ignore into journalissuearticles values(I:C); was loaded onto G 4 nanoparticles insert ignore into journalissuearticles values(PIC-G 4-DOX);. Cellular internalization study using confocal microscopy demonstrated high levels of cellular internalization of PIC-G 4-DOX nanoparticles by MCF-7 cells. This resulted in higher efficacy of PIC-G 4-DOX nanoparticles in killing MCF-7 breast cancer cells. Alteration in the expression levels of selected genes was determined by RT-qPCR analyses. Proapoptotic NOXA, PUMA, and BAX genes were upregulated, and SURVIVIN, APOLLON, and BCL-2 genes were downregulated, indicating the cell-killing effectiveness of PIC-G 4-DOX nanoparticles. Gene expression analysis provided some insights into the possible molecular mechanisms on cytotoxicity of DOX and Polyinsert ignore into journalissuearticles values(I:C); delivered through G 4 magnetic nanoparticles. The results demonstrated that PIC-G 4-DOX can be useful for targeted delivery affecting apoptotic pathways, resulting in an advanced degree of cancer-cell-killing. They are promising for targeting cancer-cells because of their stability, biocompatibility, higher internalization, and toxicity.
Keywords : Targeted drug delivery, magnetic dendrimeric nanoparticles, Poly I C, doxorubicin, gene expression