Adjuvant potency of Astragaloside VII embedded cholesterol nanoparticles for H3N2 influenza vaccine

Authors : Rukan GENC, Nilgun YAKUBOGULLARİ, Ayse NALBANTSOY, Fethiye COVEN, Erdal BEDİR

Pages : 304-314

View : 18 | Download : 7

Publication Date : 2020-12-01

Article Type : Research Paper

Abstract :Adjuvants are substances that increase the immune response to a given antigen. In the development of novel vaccine adjuvants/systems, saponins are one of the most attractive molecules due to their altered immunomodulatory activities. In this study, we tried to develop PEG insert ignore into journalissuearticles values(polyethylene glycol);/cholesterol-based lipid nanoparticles insert ignore into journalissuearticles values(LNPs); to deliver the Astragaloside VII insert ignore into journalissuearticles values(AST-VII); and potentiate adjuvant properties of AST-VII for the influenza vaccine. In the formation of PEG/cholesterol/AST-VII-based LNPs insert ignore into journalissuearticles values(PEG300: Chol-AST-VII LNPs);, 3 different primary solvents insert ignore into journalissuearticles values(acetone, ethanol, and chloroform); were evaluated, employing their effects on hydrodynamic particle size, distribution, surface chemistry, and colloidal stability. Prepared nanoparticles were simply admixtured with inactivated influenza antigen insert ignore into journalissuearticles values(H3N2); and applied to PMA insert ignore into journalissuearticles values(phorbol 12-myristate 13-acetate);-ionomycin treated human whole blood to evaluate their cytokine release profile. PEG300: Chol-AST-VII LNPs insert ignore into journalissuearticles values(80.2 +/- 7.7 nm); were obtained using chloroform as a desolvation agent. Co-treatment of PMA-ionomycin with AST-VII and PEG300: Chol-AST-VII LNPs significantly increased the levels of IL-2 and IFN-gamma, compared to PMA-ionomycin alone. In the presence of H3N2, AST-VII was able to augment IL-17A, while PEG300: Chol-AST-VII LNPs stimulated the production of IFN-gamma. Hemolysis was only observed in PEG300: Chol-AST-VII LNPs insert ignore into journalissuearticles values(250 mu g/mL); treatment. AST-VII and AST-VII-integrated LNPs could be used as efficacious adjuvants for an inactivated H3N2 vaccine in vitro, and cytokine response through Th1/Th17 route was reported.
Keywords : Immunomodulatory activity, nanocarriers, cholesterol, triterpenoid saponins, Astragaloside VII, influenza