In vitro protocorm development and mass multiplication of an endangered orchid, Dactylorhiza hatagirea

Authors : Ashish Rambhau WARGHAT, Prabodh Kumar BAJPAI, Ravi Bihari SRIVASTAVA, Om Prakash CHAURASIA

Pages : 737-746

Doi:10.3906/bot-1308-48

View : 17 | Download : 12

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Immature seeds were cultured on 10 different media for germination. Maximum germination was achieved on Lindeman orchid medium insert ignore into journalissuearticles values(37.12%); within 17 days of culturing. Protocorms with leaf primordia were cultured on BM-2 and 7 different modifications of Murashige and Skoog insert ignore into journalissuearticles values(MS); medium with various hormone combinations [0-3 mg/L indole butyric acid insert ignore into journalissuearticles values(IBA); and 0-3 mg/L kinetin insert ignore into journalissuearticles values(Kin);] for plantlet regeneration and mass multiplication. Maximum number of shoots insert ignore into journalissuearticles values(18.12 ± 0.3);, highest shoot length insert ignore into journalissuearticles values(17.80 cm ± 2.16);, maximum root number insert ignore into journalissuearticles values(8.25 ± 0.69);, and highest root length insert ignore into journalissuearticles values(8.02 cm ± 1.45); were found on MS medium with 3 mg/L IBA and 1 mg/L Kin. Plantlets with 2-3 shoots were transferred to different potting mixtures for acclimatization to field conditions and further multiplication. One hundred percent survival was obtained in C-8 potting mixture consisting of cocopeat + vermiculite + perlite insert ignore into journalissuearticles values(1:1:1);, which produced 75 shoots insert ignore into journalissuearticles values(25 plantlets); after 1 month of transplantation in the greenhouse. The current study describes for the first time a rapid in vitro protocorm development and mass multiplication protocol for Dactylorhiza hatagirea insert ignore into journalissuearticles values(D.Don); Soo that holds robust potential for large-scale propagation and metabolite production.
Keywords : Key words Conservation, development, mass multiplication, potting mixture, protocorm formation