Micropropagation of an antidiabetic medicinal plant, Artemisia pallens

Authors : Varsha Nitin NATHAR, Ghulam Mohiuddin YATOO

Pages : 491-498

Doi:10.3906/bot-1204-27

View : 18 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Artemisia pallens Wall. is an important aromatic medicinal plant used as a folk remedy for the treatment of diabetes mellitus. The present study was initiated to explore in vitro propagation of Artemisia pallens using different explants on Murashige and Skoog insert ignore into journalissuearticles values(MS); media supplemented with varying concentrations and combinations of growth regulators. Highest callogenic response insert ignore into journalissuearticles values(100%); was shown by shoot tip explants with 2 mg/L 2,4-dichlorophenoxyacetic acid. The highest number of shoots insert ignore into journalissuearticles values(14.25 ± 1.65); and highest shoot length insert ignore into journalissuearticles values(4.25 ± 0.47 cm); were observed on MS medium with 3 mg/L kinetin. After 40 days, shoots grown in vitro were transferred to rooting media. The highest number of roots insert ignore into journalissuearticles values(12 ± 0.08); and root length insert ignore into journalissuearticles values(8.15 ± 1.13 cm); were recorded on MS medium with 3 mg/L indole-3-butyric acid. The regenerated plantlets after 30 days were hardened in plastic cups containing sterile garden soil, farmyard soil, and sand insert ignore into journalissuearticles values(2:1:1); and were transferred to a greenhouse.
Keywords : Key words Acclimatization, Artemisia, callogenesis, MS medium, regeneration