New staining technique for fungal-infected plant tissues

Authors : João Paulo Rodrigues MARQUES, Marli Kasue Misaki SOARES, Beatriz APPEZZATODAGLORIA

Pages : 784-787

Doi:10.3906/bot-1204-9

View : 22 | Download : 12

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The use of histological techniques, combined with the use of dyes, facilitates the visualisation of fungi present in tissues, allowing the details of plant-pathogen interactions in many different pathosystems to be revealed. The purpose of this study is to present a new double-staining method that uses cotton blue and safranin to identify fungal structures in plant tissues. With this staining method, the fungal hyphae stain blue and plant cell walls stain orange. The new method was tested on 2 pathosystems. The technique distinguishes between fungal and plant cell walls, giving it great practical utility. This is a staining technique that can be used routinely in laboratories and facilitates the creation of permanent slides.
Keywords : Key words Citrus black spot, cotton blue, postbloom fruit drop, safranin, staining