A simple technique for cross-sectioning gymnosperm needle leaves using microtome

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Turkish Journal of Botany
Volume:36 Issue:2
A simple technique for cross-sectioning gymnosperm needle leaves using microtome

A simple technique for cross-sectioning gymnosperm needle leaves using microtome

Authors : JuKyeong EO

Pages : 213-216

View : 13 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :If a staining-permanent slide is not needed, this simple and faster microtome technique is very useful for cross-sectioning gymnosperm needle leaves. The technique employs 2× polyvinyl alcohol-lactic acid-glycerol insert ignore into journalissuearticles values(PVLG); without the steps of fixation, dehydration, clearing, embedding, deparaffinisation, and hydration. Conveniently, this technique can also be applied to thick leaves and other organs of angiosperms. The procedure is very simple and is suggested for use in plant anatomy research.
Keywords : Key words Cross section, gymnosperm, microtome, needle leaves, PVLG

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