Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species

Authors : Sandeep Kumar VERMA, Bahtiyar Buhara YÜCESAN, Günce ŞAHİN

Pages : 689-695

View : 22 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :This study reports, for the first time, an efficient in vitro plant regeneration protocol for Digitalis lamarckii Ivan. insert ignore into journalissuearticles values(dwarf foxglove); via direct shoot organogenesis. Two sets of experiments were carried out; the first compared different concentrations of 6-benzylaminopurine insert ignore into journalissuearticles values(BAP);, kinetin, thidiazuron insert ignore into journalissuearticles values(TDZ);, and zeatin alone using leaf explants excised from in vitro germinated seedlings, while the second set tested the combinations of indole-3-butyric acid insert ignore into journalissuearticles values(IBA); with BAP, kinetin, TDZ, and zeatin for shoot multiplication from the leaf explants, which were already cultured and developed numerous shoots during the first set of experiments. For shoot regeneration insert ignore into journalissuearticles values(the first set of experiments);, TDZ was the most effective at 1.0 mg L-1 concentration, producing a mean of 10.3 shoots per explant and was significantly more effective than BAP. For shoot multiplication insert ignore into journalissuearticles values(the second set of experiments);, a combination of 0.2 mg L-1 IBA with 0.2 mg L-1 TDZ produced significantly more shoots per explant insert ignore into journalissuearticles values(16.5 shoots); than with BAP insert ignore into journalissuearticles values(11.0 shoots);, zeatin insert ignore into journalissuearticles values(5.5 shoots);, or kinetin insert ignore into journalissuearticles values(4.0 shoots);. The regenerated shoots were readily rooted on medium containing 0.5 mg L-1 indole-3-acetic acid insert ignore into journalissuearticles values(IAA);. Rooted regenerants were then transferred to the pots, where they grew well and attained maturity. The described protocol provides a simple way to regenerate plants through direct shoot organogenesis, which would be useful for a large-scale production of cardenolides, germplasm conservation, and genetic transformation studies in this medicinally important endemic species.
Keywords : Key words Digitalis lamarckii, dwarf foxglove, leaf explant, direct shoot regeneration, shoot multiplication