The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey

Authors : Semiha ERİŞEN, Emine ATALAY, Mustafa YORGANCILAR

Pages : 521-526

View : 16 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Thidiazuron insert ignore into journalissuearticles values(TDZ); is a cytokinin-like substance that has often been used for shoot regeneration in recent years. We observed the effect of TDZ on callus formation and shoot regeneration from leaf and petiole explants of the endemic species Astragalus cariensis Boiss. Explants were cultured on Murashige and Skoog insert ignore into journalissuearticles values(MS); media including TDZ insert ignore into journalissuearticles values(in concentrations of 0.2, 0.4, or 0.6 mg/L); or in combination with a-naphthaleneacetic acid insert ignore into journalissuearticles values(NAA); insert ignore into journalissuearticles values(in concentrations of 0.2 or 1.0 mg/L);. Significant variations in the frequency of callus formation and the morphogenesis of the callus were obtained depending on the explant type. Petiole explant was determined to be the best for callus formation, but calli from leaf explants were more responsive in terms of shoot formation. MS media including TDZ and NAA showed 100% callus formation, but shoot regeneration was enhanced by TDZ combined with a low concentration of NAA. The highest number of shoots, insert ignore into journalissuearticles values(15 per explant); was induced from leaf explants on MS medium with 0.4 mg/L TDZ and 0.2 mg/L NAA. Regenerated shoots were rooted in MS medium containing 0.5 mg/L indole-3-butyric acid insert ignore into journalissuearticles values(IBA);. The rooted plantlets were acclimatised and produced normal plants.
Keywords : Key words Astragalus cariensis, endemic, callus, organogenesis, thidiazuron