Callus induction, biomass growth, and plant regeneration in Digitalis lanata Ehrh.: influence of plant growth regulators and carbohydrates

Authors : Zohra FATIMA, Abdul MUJIB, Samar FATIMA, Anjum ARSHI, Shahid UMAR

Pages : 393-405

View : 17 | Download : 6

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The effect of plant growth regulators insert ignore into journalissuearticles values(PGRs); and carbohydrate sources on callus induction, callus growth, and plant regeneration in foxglove was examined. Explants were transferred onto MS medium with various levels of PGRs and carbohydrates to determine the optimum explant and effective combinations of PGR treatment. For callus induction 6.0 mg L-1 of a-naphthalene acetic acid insert ignore into journalissuearticles values(NAA); and 3.0 mg L-1 of benzyl-aminopurine insert ignore into journalissuearticles values(BA); were very responsive. Addition of cytokinins insert ignore into journalissuearticles values(BA and kinetin); at 0.5-3.0 mg L-1 to media containing NAA enhanced callus growth. Shoot regeneration was best achieved in MS + 6.0 mg L-1 of BA. Adenine sulphate insert ignore into journalissuearticles values(Ade); and casein hydrolysate insert ignore into journalissuearticles values(Ch); were added to the medium as a nitrogen source to improve plant growth and maximum growth was obtained on medium supplemented with 1.5 mg L-1 of kinetin + 0.5 mg L-1 of IAA + 500 mg L-1 of Ch. Carbohydrates also influenced callus production and shoot regeneration potentiality. Among all the tested carbohydrates insert ignore into journalissuearticles values(sucrose, maltose, fructose, and glucose); and concentrations insert ignore into journalissuearticles values(3.0-6.0 g L-1);, the optimum carbohydrate concentration was 3.0 g L-1 and was applied to all carbohydrate cases.
Keywords : Key words Callus, regeneration, carbohydrates, nitrogen source, rooting