Induction of Embryogenic Tissue from Immature Zygotic Embryos in Pinus nigra J.F.Arnold subsp. nigra var. caramanica (Loudon) Businsky

Authors : Zeynep ÖZKURT, Tolga YILDIRIM, Sertaç ÖNDE, Zeki KAYA

Pages : 179-183

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Embryogenic cultures insert ignore into journalissuearticles values(EC); of Anatolian black pine insert ignore into journalissuearticles values(Pinus nigra J.F.Arnold subsp. nigra var. caramanica insert ignore into journalissuearticles values(Loudon); Businsky); were initiated from immature pre-cotyledonary zygotic embryos sampled weekly from 16 different trees in 2 consecutive years. Douglas-fir cotyledon revised insert ignore into journalissuearticles values(DCR); medium supplemented with 13.6 µM of 2,4-D and 2.2 µM of BAP was used for initiation and maintenance of EC. Overall initiation frequencies of EC in the study were 0.92% in 2004 and 1.96% in 2005; tree values ranged from 0.0% to 7.32%. Overall, 0.38% and 0.62% of the initial explants were converted into established cell lines in 2004 and 2005, respectively.
Keywords : Pinus nigra subsp nigra var caramanica, somatic embryogenesis, initiation, embryogenic cultures