Construction of a Genetic Linkage Map of Citrus with Random Amplified Polymorphic DNA (RAPD) Markers Using a Progeny Population from a Complex Intergeneric Cross

Authors : Mehtap Şahin ÇEVİK, Gloria A MOORE

Pages : 79-86

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :This study included 30 plants that were randomly selected from a larger progeny population obtained from a complex intergeneric cross of {C. grandis insert ignore into journalissuearticles values(L.); Osb. x [C. paradisi Macf. x Poncirus trifoliata insert ignore into journalissuearticles values(L.); Raf.]} x {[insert ignore into journalissuearticles values(C. paradisi Macf. x P. trifoliata insert ignore into journalissuearticles values(L.); Raf.); x C. reticulata Blanco] x [insert ignore into journalissuearticles values(C. paradisi Macf. x Poncirus trifoliata insert ignore into journalissuearticles values(L.); Raf.); x C. sinensis insert ignore into journalissuearticles values(L.); Osb.]}. Genomic DNA was extracted from leaf samples of these plants and analysed for polymorphisms by polymerase chain reaction insert ignore into journalissuearticles values(PCR);, using 10-mer random primers. A total of 111 random amplified polymorphic DNA insert ignore into journalissuearticles values(RAPD); markers were identified using 38 random primers. A genetic linkage map of the progeny population was constructed with these RAPD markers. The map contains 63 markers distributed into 9 linkage groups, which possibly correspond to the 9 haploid chromosomes of Citrus. The total maximum length of the linkage map generated in this study was 314.8 cM, with an average map distance of 5.07 cM between markers.
Keywords : Citrus, DNA markers, RAPD markers, genetic linkage mapping