Purification and Partial Characterisation of an Acid Lipase in Germinating Lipidbody Linseedlings

Authors : R H SAMMOUR

Pages : 177-184

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Electrophoretic analysis of germinating linseed proteins showed a gradual decrease in the quantity of a protein with a molecular weight of 42 kDa. This protein accumulates after 36 h of germination in synchronisation with an increase in lipase activity, and a decrease in the quantity of the total lipids. The 42 kDa subunit was found to be a lipid body membrane protein. This protein was isolated and identified by immunoprecipitation as a subunit of lipase. The linseed lipase acted on a wide range of triacylglycerols and had optimal activity at pH 4.7. The activity of the enzyme was slightly affected by a high concentration of salts and EDTA, while high concentrations of non-ionic detergents exhibited a pronounced inhibitory effect. These data suggest that the isolated 42 kDa protein is most likely a linseed acid lipase responsible for the breakdown of lipids during germination.
Keywords : Acid lipase, Triton X 100 solubilised lipid body membrane protein XLBP, ether extracted lipid body membrane protein ELBP,