In Vitro Micropropagation of Sainfoin ( Onobrychis viciifolia Scop.)

Authors : Cengiz SANCAK

Pages : 133-136

View : 21 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :A method for in vitro micropropagation of sainfoin was developed. Following optimization of the growth regulator combinations in the media, large numbers of shoots were propagated from a single embryonic axis within 8 weeks on Murashige and Skoog insert ignore into journalissuearticles values(MS); media supplemented with combination of different 6-benzylaminopurine insert ignore into journalissuearticles values(BAP);, indole-butyric acid insert ignore into journalissuearticles values(IBA); and ?-naphthaleneacetic acid insert ignore into journalissuearticles values(NAA); concentrations. The highest number of shoot multiplication was obtained on the media containing 2 mg/l BAP and either 0.05 or 0.1 mg/l IBA, and on the media supplemented with 2 mg/l BAP and either 0.05, 0.1 or 0.5 mg/l NAA, or 8 mg/l BAP and 0.05 mg/l NAA. The highest shoot length was observed on a medium containing 2 mg/l BAP only. Excised shootsrooted at a frequency of 60 % within 4 weeks in half strength MS medium containing 1 mg/l IBA and grew into normal fertile plants.
Keywords : Sainfoin, Onobrychis viciifolia, micropropagation, shoot multiplication, shoot tip culture