The assessment of genetic diversity of Castanea species by RAPD, AFLP, ISSR, and SSR markers

Authors : Sofiane ABDELHAMID, CongLinh LÊ, Marco CONEDERA, Philippe KÜPFER

Pages : 835-850

Doi:10.3906/bot-1303-30

View : 17 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Castanea Mill. insert ignore into journalissuearticles values(chestnut); is a multipurpose deciduous tree and member of the family Fagaceae, widely distributed throughout North America, Europe, and Asia. The evaluation of the genetic diversity of chestnut species is crucial for the effective conservation of this economically and ecologically valuable tree. In this study, we applied 4 DNA markers to detect the genetic variability among and within Castanea species and to compare the effectiveness of each system in estimating genetic variation. We amplified 106 random amplified polymorphic DNA insert ignore into journalissuearticles values(RAPD);, 228 amplified fragment length polymorphism insert ignore into journalissuearticles values(AFLP);, 42 intersimple sequence repeat insert ignore into journalissuearticles values(ISSR);, and 36 simple sequence repeat insert ignore into journalissuearticles values(SSR); polymorphic markers using 12, 5, 4, and 5 primer combinations, respectively. The findings on the effective multiplex ratio, polymorphism information content, and marker index revealed that AFLP was the most effective molecular marker system used in this study. Each marker system classified the species under investigation into clear but incompletely separated clusters, although partial agreement was achieved with respect to species relationships when the RAPD method was employed. The comparison of the correlation coefficient of RAPD marker data and the other markers showed a higher correlation [insert ignore into journalissuearticles values(r = 0.69, P < 0.01);, insert ignore into journalissuearticles values(r = 0.77, P < 0.01);, and insert ignore into journalissuearticles values(r = 0.47, P < 0.01); with AFLP, ISSR, and SSR, respectively]. When variance was partitioned among and within groups, AFLP insert ignore into journalissuearticles values(94.62%); showed greater variation within the groups and reverse RAPD insert ignore into journalissuearticles values(67.87%); yielded greater variation among the groups. Overall, the results indicate that the AFLP represents an efficient molecular marker system for the assessment of chestnut genetic diversity and, hence, the development of effective conservation strategies to preserve this valuable tree species.
Keywords : Castanea, DNA fingerprinting, RAPD, AFLP, ISSR, SSR, genetic diversity